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US 12,674,189 B2Granted
Use of microbial cell lines to maximize organic acid production
AssigneeJ&K Ingredients, LLC
InventorsDerrick White, James S. Brophy, Krishnamoorthy Pitchai
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Abstract
Described herein are methods, microbial cell lines, and media used in co-culture to augment propionic acid production using an optimized fermentation medium and methods for increasing propionic acid yield, e.g., by co-culturing Lacticbacillus Casei and high-acid tolerant A. Acidipropionici.
Claims20
A method for producing propionic acid, the method comprising:
- providing acid-tolerant propionic acid excreting microbes selected from the group consisting of Propionibacterium, Anaerovibrio, Bacteroides, Clostridium, Fusobacterium, Megasphaera, Propionispira, Selenomonas, Veillonella, and combinations thereof;
- providing acid-tolerant lactic acid producing microbes selected from the group consisting of Lactococcus, Pediococcus, Oenococus, Enterococcus, Leuconostoc, Bifidobacterium, Bacillus, Lactobacillus, Clostridium, Paenibacillus, Sporolactobacillus, and combinations thereof;
- providing a growth medium comprising a glucose carbon source and a lactose carbon source, and having a pH of between 5.8 and 6.5;
- adding the acid-tolerant propionic acid excreting microbes and the acid-tolerant lactic acid producing microbes to the growth medium, wherein the acid-tolerant lactic acid producing microbes are added to the growth medium 6-36 hours after the acid-tolerant propionic acid excreting microbes are added to the growth medium, thereby producing a co-culture;
- incubating the co-culture in fermentation, thereby producing propionic acid;
- and maintaining the co-culture for a time sufficient to produce a desired amount of propionic acid.
3 more elements
Dependencies are inferred from the claim text.