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US 12,674,186 B2Granted
Efficient product cleavage in template-free enzymatic synthesis of polynucleotides
AssigneeDNA Script
InventorSandrine Creton
Life of this patent
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Abstract
The present invention is directed to methods and kits for template-free enzymatic synthesis of polynucleotides that include or enable a step of efficiently cleaving the polynucleotide products from its initiator using an endonuclease V activity and initiator with a 3′-penultimate deoxyinosine.
Claims6
A kit for enzymatically synthesizing a polynucleotide comprising an initiator attached to a support by a 5′ end and having a 3′-penultimate deoxyinosine and a 3′-terminal nucleotide having a free 3′-hydroxyl.
Dependencies are inferred from the claim text.