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US 12,674,178 B2Granted
Methods for the evaluation of neutralizing antibodies for adeno-associated virus
AssigneeRegents of the University of Minnesota
InventorsGeorgiy Aslanidi, Karina Krotova
Life of this patent
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Abstract
This disclosure describes a method that includes using dorsomorphin to increase the infectivity of adeno-associated virus (AAV). The AAV may be of any AAV serotype. In some embodiments, dorsomorphin may be used in combination with IL-6 or TNFα or both. This disclosure further describes methods for using dorsomorphin-treated cells to determine neutralizing antibody (NAb) titers.
Claims17
A method comprising:
- providing an HEK293 cell responsive to an AMP-activated protein kinase (AMPK) inhibitor;
- and
- infecting the cell with adeno-associated virus (AAV) in the presence of dorsomorphin.
Dependencies are inferred from the claim text.