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US 12,674,173 B2Granted
Organelle-selective gene delivery and expression in the chloroplast in planta using chitosan-complexed single-walled carbon nanotube carriers
AssigneeMassachusetts Institute of Technology
InventorsMichael Strano, Seonyeong Kwak, Tedrick Salim Lew
Life of this patent
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Abstract
In one aspect, a composition can include an organelle, and a nanoparticle having a zeta potential of less than −10 mV or greater than 10 mV contained within the organelle. In a preferred embodiment, the organelle can be a chloroplast and the nanoparticle can be a single-walled carbon nanotube associated with a strongly anionic or strongly cationic polymer.
Claims13
A composition, comprising:
- a chitosan-complexed single-walled carbon nanotube complexed with a gene cassette for delivery to a chloroplast, wherein the chitosan-complexed single-walled carbon nanotube comprises chitosan is covalently bonded to the single-walled carbon nanotube;
- wherein the gene cassette comprises plasmid DNA;
- wherein the gene cassette and the chitosan-complexed single-walled carbon nanotube are present in a ratio of 1:3 to 1:10 w/w of gene cassette to chitosan-complexed single-walled carbon nanotube;
- and
- wherein the chitosan-complexed single-walled carbon nanotube complexed with the gene cassette has a zeta potential in the range of −35 to −30 mV or in the range of 30 to 35 mV.
2 more elements
A composition, comprising:
- a chitosan-complexed single-walled carbon nanotube complexed with a gene cassette for delivery to a chloroplast in a plant,
- wherein the chitosan-complexed single-walled carbon nanotube comprises chitosan is covalently bonded to the single-walled carbon nanotube;
- wherein the gene cassette comprises plasmid DNA;
- wherein the gene cassette and the chitosan-complexed single-walled carbon nanotube are present in a ratio of 1:3 to 1:10 w/w of gene cassette to chitosan-complexed single-walled carbon nanotube;
- and
- wherein the chitosan-complexed single-walled carbon nanotube complexed with the gene cassette has a zeta potential in the range of −35 to −30 mV or in the range of 30 to 35 mV;
- and
- wherein the plant is a watercress plant, a tobacco plant, a spinach plant or isolated Arabidopsis thaliana mesophyll protoplast.
5 more elements
Dependencies are inferred from the claim text.