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US 12,674,171 B2Granted
Aptamer and ribozyme equilibrium shifting (ARES) RNA circuits and uses thereof
AssigneeTrustees of Boston University
InventorsAlexander Arthur Green, McKayla Taylor Masity
Life of this patent
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Abstract
The technology described herein is directed to Aptamer and Ribozyme Equilibrium Shifting (ARES) regions, including ON-switches and OFF-switches, which can be harnessed to regulate the stability of RNA molecules. Also described herein are compositions comprising such RNA molecules and methods of using them to regulate translation of cargo polypeptides.
Claims22
An RNA molecule comprising:
- a) an open reading frame (ORF) encoding at least one cargo polypeptide;
- and
- b) at least one untranslated region (UTR) comprising at least one Aptamer and Ribozyme Equilibrium Shifting (ARES) region, which comprises:
- i) a protein-binding aptamer that specifically binds to a target protein;
- and
- ii) a ribozyme.
3 more elements
A self-amplifying RNA (saRNA) comprising from 5′ to 3′:
- a) a 5′ conserved sequence element (CSE);
- b) a first open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRP), operably linked to a promoter in the 5′ CSE;
- c) a subgenomic promoter (SGP);
- d) a second ORF encoding at least one cargo polypeptide, operably linked to the SGP;
- and
- e) a 3′ CSE;
- and
- wherein the 5′ CSE, a region between the first and second ORFs, and/or the 3′ CSE further comprises at least one Aptamer and Ribozyme Equilibrium Shifting (ARES) region in a sense or anti-sense orientation, wherein the ARES region comprises:
- i) a protein-aptamer that specifically binds to a target protein;
- and
- ii) a ribozyme.
8 more elements
Dependencies are inferred from the claim text.