Transport media for clinical specimen collection and molecular diagnostic applications and methods of production and use thereof
AssigneeSiemens Healthcare Diagnostics Inc.
InventorsAlex Hofai Lee, Qi Dang, Jocelyn Lee
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Abstract
A transport medium is disclosed that can be utilized for both sample collection and molecular diagnostic applications. The transport medium can be utilized with multiple types of biological samples and maintains the stability of nucleic acid present in the biological samples so that one or more nucleic acid assay target(s) present in the biological sample is not substantially degraded during storage and shipping. Also disclosed are kits containing the transport medium, mixtures that include a biological sample disposed in the transport medium, and methods of producing and using the medium.
Claims13
A transport medium for sample collection and molecular diagnostic applications, the transport medium comprising:
- guanidine thiocyanate in a sodium acetate buffer, wherein the guanidine thiocyanate is present at a concentration in a range of from about 3M to about 4M;
- a polysorbate, wherein the polysorbate is present at a concentration in the range of from about 0.1% to about 20%;
- CHAPS (3-((3-cholamidopropyl)dimethylammonio)-1-propanesulfonate), wherein CHAPS is present at a concentration in a range of from about 0.1% to about 5%;
- EDTA (ethylenediaminetetraacetic acid);
- an alcohol, wherein the alcohol is present at a concentration in a range of from about 1% to about 40%;
- and
- at least one reducing agent selected from the group consisting of TCEP (Tris(2-carboxyethyl) phosphine)), DTT (dithiothreitol), and a combination thereof, wherein the transport medium substantially preserves DNA and/or RNA from the biological sample for a period of at least about 15 days to 30 days at room temperature.
Dependencies are inferred from the claim text.