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US 12,674,144 B2Granted
Method of producing a mesodermal-lineage primitive streak cell
AssigneeKyoto University
InventorsShinichi Sueta, Tomoko Kasahara, Kenji Osafune
Life of this patent
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Abstract
A method of producing a mesodermal-lineage primitive streak cell includes a first step of culturing pluripotent stem cells in a medium containing FGF2, BMP (bone morphogenetic protein) 4, a GSK-3β inhibitor, and retinoic acid or a derivative thereof and a second step of culturing cells obtained in the first step in a medium containing FGF2, a GSK-3β inhibitor, and BMP7.
Claims7
A method of producing a mesodermal-lineage primitive streak cells comprising:
- (i) culturing pluripotent stem cells in a medium containing fibroblast growth factor 2 (FGF2), bone morphogenic protein (BMP) 4, a glycogen synthase kinase-3β (GSK-3β) inhibitor, and retinoic acid or a derivative thereof;
- and
- (ii) culturing cells obtained in (i) in a medium containing FGF2, a GSK-3β inhibitor, and BMP7 thereby producing the mesodermal-lineage primitive streak cell.
Dependencies are inferred from the claim text.