Serum-free medium not containing albumin and suited for culturing human hematopoietic stem cells, and albumin-free culturing method
AssigneeThe University of Tokyo
InventorsSatoshi Yamazaki, Motoo Watanabe, Masatoshi Sakurai, Hiromitsu Nakauchi
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Abstract
The present invention discloses a composition of an albumin-free, serum-free medium suited for culturing human hematopoietic stem cells and an albumin-free culturing method. According to the present invention, a method of culturing human hematopoietic stem cells is provided which comprises bringing human hematopoietic stem cells into contact with PVA and a PI3K activator.
Claims13
A method of culturing or manufacturing human hematopoietic stem cells,
- the method comprising culturing human hematopoietic stem cells in a culture medium,
- the culture medium comprising polyvinyl alcohol,
- (1) comprising a phosphatidylinositol 3-kinase (PI3K) activator and one or more selected from the group consisting of thrombopoietin (TPO) and a TPO receptor agonist;
- (2) comprising one or more selected from the group consisting of a stem cell factor (SCF) and a PI3K activator, and a TPO receptor agonist;
- or
- (3) comprising a PI3K activator and a TPO receptor agonist,
- wherein the PI3K activator is 740Y-P,
- wherein the TPO receptor agonist is butyzamide, and
- wherein the number of human hematopoietic stem cells is increased by the culturing.
A method of culturing or manufacturing human hematopoietic stem cells, the method comprising culturing human hematopoietic stem cells in a culture medium comprising polyvinyl alcohol as an albumin replacement,
- (1) a PI3K activator and one or more selected from the group consisting of TPO and a TPO receptor agonist;
- (2) one or more selected from the group consisting of SCF and a PI3K activator, and a TPO receptor agonist;
- or
- (3) a PI3K activator and a TPO receptor agonist,
- wherein the PI3K activator is 740Y-P, and
- wherein the TPO receptor agonist is butyzamide.
Dependencies are inferred from the claim text.