Three-dimensional culture of immortalized skin cells on the superficial layer of which a stratum corneum is formed, method for producing said three-dimensional culture, and method for evaluating test substance using said three-dimensional culture

Abstract

Provided is a three-dimensional culture of immortalized skin cells, comprising a plate-like substrate and a culture layered product of immortalized skin cells adhering to the surface of the plate-like substrate, in which the superficial layer of the culture layered product is composed of a stratum corneum. The three-dimensional culture can be produced as follows: immortalized skin cells are seeded on a top surface of a culture medium-impregnated plate-like substrate, and three-dimensional culture is performed while applying stress on a superficial layer of an immortalized skin cell layer formed by layering of the immortalized skin cells. The three-dimensional culture can be used to evaluate the sensitization of a test substance.

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