Live attenuated non-transmissible vaccines
AssigneeUniversity of Maryland, Baltimore
InventorsSharon Tennant, James Eugene Galen
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Abstract
The present invention provides genetically modified microorganisms with reduced transmissibility, wherein the microorganism has been genetically modified to express a single stranded binding protein (SSB) regulated by an arabinose responsive promoter, wherein the microorganism has a reduced growth capacity in the absence of arabinose.
Claims18
A genetically modified Salmonella enterica microorganism having reduced fecal-oral transmission, wherein the microorganism has been genetically modified to express a single stranded binding protein (SSB) regulated by a promoter that is responsive to arabinose, wherein the Salmonella enterica microorganism has a reduced growth capacity in the absence of arabinose, wherein the promoter is a PBAD promoter, wherein the microorganism has been further modified to reduce expression of endogenous AraB, AraA, and/or AraD, wherein an endogenous araBAD operon is deleted in the microorganism.
Dependencies are inferred from the claim text.