Miniaturized centrifugal bioreactor and rotor system

A centrifugal bioreactor comprising:

• a turntable assembly;
• an electric motor coupled to the turntable assembly;
• a replaceable rotor assembly coupled to the turntable assembly, the replaceable rotor assembly including a central disc that forms (i) a plurality of reaction chambers configured to house cell cultures and (ii) conduits configured to provide fluid routing to and from the plurality of reaction chambers, respectively;
• a fresh medium tank configured to hold fresh medium for the cell cultures;
• a spent medium tank configured to hold a spent medium produced by the cell cultures;
• an inoculation tank configured to combine the fresh medium with a portion of the spent medium;
• a gas exchanger configured to expose the fresh medium and the portion of the spent medium to gas;
• a first pump fluidly connected to the fresh medium tank, the inoculation tank, and the replaceable rotor assembly, wherein individual reaction chambers of the plurality of reaction chambers are in direct fluid connection with the first pump;
• and
• a second pump fluidly connected to the replaceable rotor assembly and the spent medium tank.