Systems and methods for cell culturing

A cell culture method, the method comprising:

• providing a cell culture system comprising a plurality of shelves stacked with a first shelf on top of a second shelf, each of the first and second shelves configured to receive a fluid reservoir;
• at least one pump;
• a processor operably coupled to the at least one pump;
• a platform supporting a substrate comprising a plurality of openings sized and configured to simultaneously receive and retain at least two interchangeable cell culture chambers in a respective position onto said platform, and a central processing unit (CPU) comprising memory containing instructions executable by the CPU to cause the system to:
• receive, as a first input, data comprising a capacity of the first and/or second cell culture chamber;
• receive, as a second input, data comprising a first concentration of a first cell type and a second concentration of a second cell type in one or more fluids to be introduced into the first and/or second cell culture chamber;
• and
• calculate, based on the first and second inputs, a perfusion rate of a perfusion fluid to be introduced into the first and/or second cell culture chamber that maximizes a probability of the first cell type and the second cell type contacting each other within the cell culture chamber;
• loading a first fluid reservoir onto the first shelf;
• loading a second fluid reservoir onto the second shelf;
• loading a first cell culture chamber and a second cell culture chamber onto the substrate;
• connecting the first fluid and second fluid reservoirs, the at least one pump, and the first and second cell culture chambers, with tubing;
• and
• operating the system to culture cells inside the first and second cell culture chambers.