Fluidic system for producing extracellular vesicles and associated method

A method for ex vivo production of extracellular vesicles from suspended producer cells, comprising:

• controlling the speed of an agitator adapted for the growth of the suspended producer cells,
• wherein the producer cells are non-adherent cells or adherent cells detached from a culture support,
• wherein a container has a shape and dimensions adapted to the generation of a turbulent flow of a liquid medium in the container to exert shear stresses on the producer cells in order to achieve the production of extracellular vesicles, the flow having a Kolmogorov length less than or equal to 50 μm in the container, the container comprising an output, the liquid medium comprising suspended producer cells, and
• collecting the liquid medium comprising extracellular vesicles at the output of the container.