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US 12,674,059 B2Granted
Violet excitable tandem dyes, and methods for making and using the same
AssigneeBecton, Dickinson and Company
InventorsGlenn Bartholomew, David Michel Moureau, Itzel Vaca
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Abstract
Violet excitable tandem dyes are provided. Tandem dyes of embodiments of the invention include: a non-conjugated polymeric backbone; one or more violet excitable donor fluorophores linked to the non-conjugated polymeric backbone; and one or more acceptor fluorophores linked to the non-conjugated polymeric backbone; where the donor and acceptor fluorophores are in energy transfer relationship. Also provided are methods of making and using the tandem dyes, as well as kits that include the dyes and find use in embodiments of the methods.
Claims19
A tandem dye comprising:
- a non-conjugated polymeric backbone;
- one or more violet excitable donor fluorophores selected to have an absorption maximum ranging from 375 to 425 nm linked to the non-conjugated polymeric backbone;
- and
- one or more acceptor fluorophores linked to the non-conjugated polymeric backbone,
- wherein the donor and acceptor fluorophores are in energy transfer relationship.
2 more elements
Dependencies are inferred from the claim text.