Protein containing heterodimer antibody Fc, and preparation method therefor

A heterodimeric Fc scaffold, comprising:

• a first Fc region comprising a first CH3 region, and a second Fc region comprising a second CH3 region, wherein the first CH3 region and the second CH3 region are different in sequence, wherein the heterodimeric interaction between the first CH3 region and the second CH3 region is stronger than the homodimeric interactions of the first CH3 region and the second CH3 region;
• wherein, compared to a parent CH3 region, the first CH3 region comprises S364R and D399K, and the second CH3 region comprises K370S, Y349T and K409D, based on the EU numbering system;
• and
• wherein the parent CH3 region is a CH3 region from wild type human IgG1.