Microbial inoculant for high-speed humification of organic waste and preparation method thereof

A preparation method of a microbial inoculant for a high-speed humification of an organic waste, comprising the following steps:

• step 1, initial culturing by preparing a culture medium, and adding albite beads, Thermus tengchongensis GT2, and Thermus amyloliquefaciens GT3 to the culture medium;
• culturing at 55-85° C. for 24-128 h, energizing the culture medium at a weak current of 5-15 mA, and connecting a negative electrode, to make the Thermus tengchongensis GT2 and the Thermus amyloliquefaciens GT3 gather to the albite beads;
• step 2, membrane-covered enrichment culturing by adding 10-50 mg/L Fe3+ and Geobacillus stearothermophilusGT1 to the culture medium, and culturing at 65-75° C. for 5-72 h;
• step 3, membrane removing by taking out the albite beads, putting the albite beads into an electrolysis unit, and treating for 0.5-2 h at a current of 16-25 A to obtain a biofilm;
• and
• step 4, preparing the microbial inoculant by adding 5-15% of activated carbon particles to the biofilm, and drying or freeze-drying to obtain the microbial inoculant;
• wherein proteins, complex polysaccharides, or simple polysaccharides in the organic waste account for ≥60% of organic matter, the complex polysaccharides are selected from the group consisting of cellulose and lignin, and the simple polysaccharides are selected from the group consisting of starch and sucrose;
• and
• the Geobacillus stearothermophilusGT1 is a Geobacillus stearothermophilus strain deposited at the China General Microbiological Culture Collection Center (CGMCC) on Jul. 19, 2021, with an accession number of CGMCC No. 22925;
• the Thermus tengchongensis GT2 is a Thermus tengchongensis strain deposited at the CGMCC on Jul. 19, 2021, with an accession number of CGMCC No. 22927;
• and the Thermus amyloliquefaciens GT3 is a Thermus amyloliquefaciens strain deposited at the CGMCC on Jul. 19, 2021, with an accession number of CGMCC No. 22928.