Dynamic antimicrobial hydrogel based on natural receptor-ligand recognition, and preparation method and use thereof

A preparation method of a dynamic hydrogel based on a natural receptor-ligand recognition, comprising the following steps:

• (1) modification of a ligand vancomycin by dissolving vancomycin hydrochloride and a modification material in an organic solution or pure water to obtain a first resulting solution, introducing an inert gas in the first resulting solution to remove oxygen, and adjusting a pH of the first resulting solution to obtain a second resulting solution;
• conducting a hydroxyl-carboxyl esterification reaction, an amino-carboxyl amidation reaction, and a transesterification reaction on the second resulting solution at a constant temperature to obtain a first resulting reaction system, and after the hydroxyl-carboxyl esterification reaction, the amino-carboxyl amidation reaction, and the transesterification reaction, subjecting the first resulting reaction system to a dialysis to obtain Van-M;
• and lyophilizing the Van-M to obtain a first lyophilized product, and storing the first lyophilized product at −20° C. for later use;
• (2) modification of a receptor AA-based (dipeptide D-Ala-D-Ala based) material by dissolving the receptor AA-based material and the modification material in a mixed solution of the organic solution and water to obtain a third resulting solution, introducing the inert gas in the third resulting solution to remove oxygen, and adjusting a pH of the third resulting solution to obtain a fourth resulting solution;
• conducting the hydroxyl-carboxyl esterification reaction, the amino-carboxyl amidation reaction, the transesterification reaction, and a mercapto-alkenyl addition reaction on the fourth resulting solution at the constant temperature to obtain a second resulting reaction system, and subjecting the second resulting reaction system to the dialysis to obtain AA-M;
• and lyophilizing the AA-M to obtain a second lyophilized product, and storing the second lyophilized product at −20° C. for later use;
• and
• (3) preparation of a hydrogel material by dissolving the Van-M obtained in the step (1) and the AA-M obtained in the step (2) in the water to obtain a fifth resulting solution, and adding a photoinitiator in the fifth resulting solution to trigger a crosslinking reaction under irradiation of ultraviolet light to obtain the dynamic hydrogel.