Engineered probiotics for expression of fiber-synthesizing enzymes in gut
AssigneeZBiotics Company
InventorsZachary D. Abbott, John William Kidder Oliver
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Abstract
The present disclosure provides solutions to various challenges in health, including health challenges related to high consumption of carbohydrate and/or low consumption of soluble fiber. The present disclosure provides, among other things, bacteria engineered for expression of a fiber-synthesizing enzyme, e.g., for expression of the enzyme in the gut (e.g., in the intestine). In particular embodiments, the present disclosure provides bacteria engineered for expression of a fiber-synthesizing enzyme that consumes carbohydrate in the process of synthesizing fiber, e.g., in the gut. The present disclosure further includes formulations of isolated fiber-synthesizing enzymes for administration to subjects.
Claims75
A method of decreasing the amount or concentration of a carbohydrate in the gut of a subject comprising delivering to the gut of to the subject a bacterium engineered to express an integrated heterologous nucleic acid sequence encoding a fiber-synthesizing enzyme, wherein the fiber-synthesizing enzyme synthesizes fiber from a carbohydrate substrate, where in the enzyme is not a trehalose-6-phosphate synthase and the fiber is not trehalose.
A method of increasing the amount or concentration of a fiber in the gut of a subject comprising delivering to the gut of to the subject a bacterium engineered to express an integrated heterologous nucleic acid sequence encoding a fiber-synthesizing enzyme, wherein the fiber-synthesizing enzyme synthesizes fiber from a carbohydrate substrate, where in the enzyme is not a trehalose-6-phosphate synthase and the fiber is not trehalose.
A method of treating a subject in need of decreased amount or concentration of a carbohydrate in the gut or increased amount or concentration of a fiber in the gut comprising delivering to the gut of to the subject a bacterium engineered to express an integrated heterologous nucleic acid sequence encoding a fiber-synthesizing enzyme, wherein the fiber-synthesizing enzyme synthesizes fiber from a carbohydrate substrate, where in the enzyme is not a trehalose-6-phosphate synthase and the fiber is not trehalose.
Dependencies are inferred from the claim text.