Identification, quantitation and analysis of unique biomarkers in sweat

A diagnostic method comprising:

• non-invasively collecting at least one sweat sample from a biological subject undergoing physical activity and storing said sweat samples for a later analysis;
• performing metabolic and proteomic profiling of biomarkers in the collected sweat sample;
• comparing the metabolic and proteomic profile with a predetermined profile of the biomarkers;
• determining a first physiological status of the of the biological subject from the biomarkers based on the comparison between the metabolic and proteomic profiles and predetermined profile of biomarkers;
• comparing the first physiological status of the of the biological subject determined from the at least one sweat sample from the biological subject to a second physiological status of the of the biological subject determined from at least a second sweat sample.