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US 12,672,795 B2Granted
Capturing truncated proteoforms in exhaled breath for diagnosis of tuberculosis
AssigneeZeteo Tech, Inc.
InventorsDapeng Chen, Wayne A. Bryden, Michael McLoughlin, Kiana M. Kiser, Emily R. Caton, Caroline R. Haddaway
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Abstract
Methods and devices to capture and analyze aerosolized particles in exhaled air including protein biomarkers and their truncated proteoforms characteristic of tuberculosis to enable rapid detection of diseases. Methods and systems to selectively capture aerosolized particles using a packed bed column. The captured particles are eluted using one or more solvents and analyzed using devices including MALDI-TOFMS.
Claims15
A method for detecting tuberculosis (TB) using exhaled air, the method including:
- capturing truncated proteoforms in the exhaled air aerosol produced by a patient using a sample capture element including a packed bed column, wherein exhaled air is drawn into the sample capture element using a pump at a predetermined flow rate;
- extracting the truncated proteoforms from the packed bed column into one or more collected liquid samples including the truncated proteoforms;
- analyzing the one or more collected liquid samples using MALDI-TOFMS;
- detecting the presence of TB if the truncated proteoforms includes one or more of interleukin-10 receptor subunit alpha (IL10RA), protein phosphatase 1 regulatory subunit 17 (PPR17), collagen alpha-1 (II) chain (COL2A1), collagen alpha-1 (III) chain (COL3A1), or complement component C6 (C6).
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Dependencies are inferred from the claim text.