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US 12,669,468 B2Granted
Capillary-array-electrophoresis device
AssigneeHitachi High-Tech Corporation
InventorsTakashi Anazawa, Ryoji Inaba, Shuhei Yamamoto
Life of this patent
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Abstract
When laser-irradiation portions of N capillaries with capillary numbers n=1, 2, . . . , and N are arranged on a same plane, a laser-irradiation intensity of each capillary is denoted by L(n), and an output intensity of each capillary by a computer when a light emitting substance having an equal concentration exists inside each capillary is denoted by H(n), an absolute value of an average value of a second derivative of H(n) becomes smaller than an absolute value of an average value of a second derivative of L(n) for any refractive index n3 of a separation medium in a range of 1.33≤n3≤1.41 by digital correction by the computer, which changes according to the refractive index n3 (see FIG. 11).
Claims14
A capillary-array-electrophoresis device comprising:
- a laser-light source configured to emit a laser beam;
- a capillary array configured such that laser-irradiation portions of N capillaries collectively irradiated with the laser beam are substantially arranged on a same array plane, where N is an integer of 2 or more;
- an optical system configured to collectively measure light emission from the N capillaries;
- and
- a computer configured to perform predetermined processing on light intensities measured by the optical system and to output the processed light intensities,
- wherein at least an analysis mode of capillary electrophoresis using a separation medium having a refractive index n3<1.36 is provided, |A|>|B| is satisfied,
- wherein an outer radius, an inner radius, a refractive index of an external medium, a refractive index of a material, and a refractive index of an internal medium of the N capillaries in the laser-irradiation portions are respectively defined as R, r, n1, n2, and n3,
- wherein the N capillaries are numbered n=1, 2, . . . , and N in order from the capillary at the end of the capillary array,
- wherein an average value of a second derivative of L(n) in the range of 1≤n≤N is defined as A, wherein L(n) represents a laser-irradiation light intensity of the capillary with capillary number n as a function of n, and A indicates a degree of convexity of L(n) and
- wherein an average value of a second derivative of H(n) in the range of 1≤n≤N is defined as B, wherein H(n) represents an output light intensity of the capillary with capillary number n by the computer as a function of n when a light emitting substance of equal concentration exists inside the N capillaries in the laser-irradiation portions, and B indicates a degree of convexity of H(n).
7 more elements
A capillary-array-electrophoresis device comprising:
- a laser-light source configured to emit a laser beam;
- a capillary array configured such that laser-irradiation portions of N capillaries collectively irradiated with the laser beam are substantially arranged on a same array plane, where N is an integer of 2 or more;
- an optical system configured to collectively measure light emission from the N capillaries;
- and
- a computer configured to execute predetermined processing on light intensity data measured by the optical system,
- wherein the computer is configured to change by the predetermined processing, a sign of an average value of a second derivative of H(n) in the range of 1≤n≤N from positive to negative with an increase of a refractive index n3,
- wherein an outer radius, an inner radius, a refractive index of an external medium, a refractive index of a material, and a refractive index of an internal medium of the N capillaries in the laser-irradiation portions are respectively defined as R, r, n1, n2 and n3,
- wherein the N capillaries are numbers n=1, 2, . . . , and N in order from the capillary at the end of the capillary array,
- wherein H(n) represents an output light intensity of the capillary number n by the computer as a function of n when a light emitting substance of equal concentration exists inside the N capillaries in the laser-irradiation portions.
6 more elements
A capillary-array-electrophoresis device comprising:
- a laser-light source configured to emit a laser beam;
- a capillary array configured such that laser-irradiation portions of N capillaries collectively irradiated with the laser beam are substantially arranged on a same array plane, where N is an integer of 2 or more;
- an optical system configured to collectively measure light emission from the N capillaries;
- and
- a computer configured to output light intensities measured by the optical system,
- wherein a separation medium injected into the capillary array is selected from a plurality kinds of separation media having different refractive indexes in a range of 1.33≤n3≤1.41,
- wherein an outer radius, an inner radius, a refractive index of an external medium, a refractive index of a material, and a refractive index of an internal medium of the N capillaries in the laser-irradiation portions are respectively defined as R, r, n1, n2, and n3, and
- wherein the N capillaries are numbers n=1, 2, . . . , and N in order from the capillary at the end of the capillary array, and
- wherein the computer is configured to display a user interface screen for selecting a separation medium to be used from the plurality kinds of separation media on a display screen.
6 more elements
Dependencies are inferred from the claim text.