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US 12,669,416 B2Granted
Methods and devices for manipulating biological samples
AssigneeSingular Genomics Systems, Inc.
InventorsYuji Ishitsuka, Jaekyung Koh, Robert James Stover, Pavel Shekhtmeyster, Sandor Kovacs, Hu Cang, Valeria Rascon
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Abstract
Disclosed herein, inter alia, are devices and methods for efficient transfer and analyses of cellular material, tissue samples, such as tissue sections, using carrier substrates and devices.
Claims23
A method for manipulating a biological sample, comprising:
- positioning a punch device against a sample-carrier construct formed of a tissue section and carrier substrate, wherein the tissue is mounted on the carrier substrate, the punch device formed of an outer wall having a top edge, a bottom edge, and an internal lumen;
- pushing the bottom edge of the punch device through the sample-carrier construct to cut through the sample-carrier construct such that an excised tissue section is positioned within the internal lumen of the punch device to form a loaded punch device;
- positioning the loaded punch device against a slide;
- positioning a piston relative to the loaded punch device so that the piston can be inserted into the loaded punch device;
- and
- using the piston to push the excised tissue section out of the loaded punch device toward the slide.
3 more elements
A method for manipulating a biological sample, comprising:
- positioning a loaded punch device against a glass solid support, wherein said loaded punch device comprises an outer wall having a top edge, a bottom edge, and an internal lumen, wherein a tissue sample is positioned within the internal lumen;
- inserting a plunger through the top edge into the internal lumen and advancing the plunger through the internal lumen to push the tissue sample to contact the glass solid support;
- and
- while the tissue sample remains in contact with the glass solid support, heating the glass solid support to a temperature of 30° C. or greater.
1 more element
Dependencies are inferred from the claim text.