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US 12,668,834 B2Granted
Strand displacing amplification enzymes
AssigneeSingular Genomics Systems, Inc.
InventorsSouad Naji, Eli N. Glezer, Bruna Krebs Kutche, Ali Nikoomanzar, Alexander Sevy, Caleb Newman, Ryan Shultzaberger, Abrehet Abdu, Youngjin Cho, Janell J. Kosmatka
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Abstract
Disclosed herein, inter alia, are novel strand-displacing polymerases and methods of use thereof.
Claims21
A polymerase comprising an amino acid sequence that is at least 80% identical to a contiguous 500 amino acid sequence within SEQ ID NO:
- 1;
- comprising
- a first substitution at amino acid position 588 or an amino acid position corresponding to position 588;
- wherein the first substitution is leucine, isoleucine, valine, alanine, or glycine;
- and
- a second substitution:
- at amino acid position 7 or an amino acid position corresponding to position 7, wherein the second substitution is histidine, lysine, or arginine;
- at amino acid position 97 or an amino acid position corresponding to position 97, wherein the second substitution is cysteine, histidine, lysine, serine, threonine, or methionine;
- or
- at amino acid position 742 or an amino acid position corresponding to position 742, wherein the second substitution is leucine, isoleucine, alanine, or glycine.
7 more elements
Dependencies are inferred from the claim text.