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US 12,668,824 B2Granted
Engineered transaminase biocatalysts and methods for synthesizing chiral amines
AssigneeCodexis, Inc.
InventorsSanthosh Sivaramakrishnan, Ericka Bermudez, Nandhitha Subramanian, David Entwistle, Stephanie Marie Forget
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Abstract
The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents.
Claims9
An engineered transaminase comprising a polypeptide sequence having at least 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, sequence identity to SEQ ID NO:
- 6, or a functional fragment thereof, wherein said engineered transaminase comprises a 130Y substitution in said polypeptide sequence, and wherein the amino acid positions of said polypeptide sequence are numbered with reference to SEQ ID NO:
- 6, and wherein the engineered transaminase has an increased activity as compared to the polypeptide of SEQ ID NO:
- 4 in converting 1-imidazo[1,2-a]pyridin-6-ylethanone (compound (2)) to (1S)-1-imidazo[1,2-a]pyridin-6-ylethanamine (compound (3)).
Dependencies are inferred from the claim text.