Enzymatic production of glucaric acid from glucuronic acid
AssigneeThe Governing Council of the University of Toronto
InventorsEmma R. Master, Thu V. Vuong
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Abstract
Described herein are processes and enzymes for the enzymatic production of glucaric acid from glucuronic acid, and more specifically the enzymatic production of substituted glucaric acid from substituted glucuronic acid. Advantageously, the process and enzymes described herein make preferential use substituted glucuronic acid obtained from natural sources, such as underutilized hemicellulose stream from wood and agricultural biorefineries, to produce substantially enantiomerically pure substituted D-glucaric acid.
Claims17
A process for producing 4-O-methyl glucaric acid, comprising:
- providing a solution comprising dissolved 4-O-methyl glucuronic acid;
- providing a recombinant gluco-oligosaccharide oxidase (GOOX) having at least 99% sequence identity to SEQ ID NO:
- 1;
- and
- contacting the dissolved 4-O-methyl glucuronic acid with said recombinant GOOX under conditions that catalyze enzymatic conversion of the 4-O-methyl glucuronic acid to 4-O-methyl glucaric acid.
A composition comprising a recombinant gluco-oligosaccharide oxidase (GOOX) having at least 99% sequence identity to SEQ ID NO:
- 1, O-methyl glucuronic acid as a GOOX substrate, and an alkaline reaction buffer enabling enzymatic conversion of the 4-O-methyl glucuronic acid to 4-O-methyl glucaric acid.
Dependencies are inferred from the claim text.