Microorganisms and methods for enhancing the availability of reducing equivalents in the presence of methanol, and for producing 1,4-butanediol related thereto
AssigneeGenomatica, Inc.
InventorsAnthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Cara Ann Tracewell, Priti Pharkya, Stefan Andrae
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Abstract
Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway (MMP) that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,4-butanediol (BDO). Also provided herein are methods for using such an organism to produce BDO.
Claims19
A non-naturally occurring microbial organism (NNOMO) having a methanol metabolic pathway (MMP), wherein said organism comprises at least two exogenous nucleic acids encoding MMP enzymes (MMPE) expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of methanol, wherein said MMP comprises:
- (i) a methanol dehydrogenase (EM9) and a formaldehyde activating enzyme (EM10), wherein EM9 converts methanol to formaldehyde and wherein EM10 converts formaldehyde to methylene-THF;
- and
- (ii) a methanol methyltransferase (EM1) and a methylenetetrahydrofolate reductase (EM2), wherein EM1 converts methanol to methyl-THF and EM2 converts methyl-THF to methylene-THF.
Dependencies are inferred from the claim text.