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US 12,668,819 B2Granted
CRISPR-aid using catalytically inactive RNA-guided endonuclease
AssigneeNovozymes A/S
InventorsTakashi Nakanishi, Michael David Thomas
Life of this patent
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Abstract
The present invention relates to nucleobase editing complexes comprising a) a catalytically inactive RNA-guided endonuclease having a sequence identity of at least 60% to SEQ ID NO: 126 or SEQ ID NO: 155 and b) a nucleobase editing domain, as well as polynucleotides encoding said nucleobase editing complexes, nucleic acid constructs and expression vectors comprising said polynucleotides, host cells comprising said nucleobase editing complexes and/or polynucleotides, and methods for preparing and using said nucleobase editing complexes.
Claims34
A nucleobase editing complex comprising:
- a) a catalytically inactive RNA-guided endonuclease having a sequence identity of at least 90% to SEQ ID NO:
- 126;
- and
- b) a nucleobase editing domain;
- wherein the catalytically inactive RNA-guided endonuclease and the nucleobase editing domain are fused end-to-end or connected via a linker polypeptide.
2 more elements
Dependencies are inferred from the claim text.