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US 12,668,810 B2Granted
Exon-humanized mouse
AssigneeTransgenic Inc.
InventorsKenichi Yamamura, Zhenghua Li
Life of this patent
Estimating the term…
Abstract
Provided are a donor vector having an exon-humanized gene in which only exon nucleotide sequences of a mouse gene are replaced with human exon nucleotide sequences, an ES cell in which a mouse endogenous gene is replaced with the donor vector, and a mouse crated by using the ES cell.
Claims6
A donor vector comprising a fragment of a target gene, wherein the fragment of the target gene comprises four exons and three introns, wherein the four exons are human nucleotide sequences of the target gene as set forth in SEO ID NOs:
- 4, 6, 8, and 10, and the three introns are mouse nucleotide sequences as set forth in SEO ID NOs:
- 5, 7, 9 wherein the target gene is a transthyretin gene, wherein the four exons and the three introns are arranged in the following order;
- Exon 1 as set forth in SEQ ID NO:
- 4, Intron 1 as set forth in SEQ ID NO:5, Exon 2 as set forth in SEQ ID NO:6, Intron 2 as set forth in SEQ ID NO:7, Exon 3 as set forth in SEQ ID NO:8, Intron 3 as set forth in SEQ ID NO:9, and Exon 4 as set forth in SEQ ID NO:
- 10.
2 more elements
Dependencies are inferred from the claim text.