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US 12,668,792 B2Granted
Oligonucleotide replacement for di-tagged and directional libraries
AssigneeIllumina, Inc.
InventorsIgor Goryshin, Bradley Baas, Ramesh Vaidyanathan, Mark Maffitt
Life of this patent
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Abstract
Transposomes and oligonucleotide replacement methods to make DNA libraries that have distinct 5′ and 3′ tags, and to make directional libraries that are enriched for a desired strand.
Claims20
An array, comprising:
- a surface of the array, the surface comprising a plurality of target nucleic acids;
- and
- a plurality of transposomes bound to the plurality of target nucleic acids, wherein a transposome of the plurality of transposomes comprises:
- a tag sequence;
- a transposase;
- and
- one or two copies of a transposase end sequence selected from the group consisting of:
- (SEQ ID NO:
- 1)
- MRWTGTGHWKAVGARACAV
- and
- (SEQ ID NO:
- 2)
- NSHBGHSHDDRNGAKACAN.
11 more elements
An array surface, comprising:
- a plurality of target nucleic acids disposed in chambers of the array surface;
- and
- a plurality of transposomes bound to the plurality of target nucleic acids, wherein a transposome of the plurality of transposomes comprises:
- a tag sequence;
- a transposase;
- and
- a transposase end sequence, the transposase end sequence comprising one or two copies of a sequence comprising:
- (SEQ ID NO:
- 1)
- MRWTGTGHWKAVGARACAV;
- or
- (SEQ ID NO:
- 2)
- NSHBGHSHDDRNGAKACAN;
- but excluding
- (SEQ ID NO:
- 3)
- AGATGTGTATAAGAGACAG.
15 more elements
An array surface, comprising:
- a plurality of target nucleic acids;
- and
- a plurality of transposomes bound to the plurality of target nucleic acids, wherein a transposome of the plurality of transposomes comprises:
- a transposase;
- and
- a double-stranded nucleic acid, the double-stranded nucleic acid comprising a first strand and a complementary second strand, the first strand comprising:
- (SEQ ID NO:
- 1)
- MRWTGTGHWKAVGARACAV;
- or
- (SEQ ID NO:
- 2)
- NSHBGHSHDDRNGAKACAN;
- but excluding
- (SEQ ID NO:
- 3)
- AGATGTGTATAAGAGACAG.
14 more elements
Dependencies are inferred from the claim text.