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US 12,668,790 B2Granted
Lysis, binding and/or wash reagent for isolating and/or purifying nucleic acids
AssigneeQiagen GmbH
InventorsRoland Fabis, Anke Homann, Thorsten Voss, Thomas Hanselle
Life of this patent
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Abstract
The present invention relates to a lysis, binding and/or wash reagent for isolating and/or purifying nucleic acids and a method for isolating and/or purifying nucleic acids.
Claims14
A lysis, binding or wash reagent comprising:
- at least one chaotropic compound that is a guanidinium salt,
- at least one buffer compound, and
- a polyoxyethylene-based non-ionic surfactant consisting essentially of polyoxyethylene (5) nonylphenylether, polyoxyethylene (9) nonylphenylether, or polyoxyethylene (9 or 10) isooctylphenylether, in the range from ≥8% weight/volume to ≤50% weight/volume based on the total volume of the reagent.
Dependencies are inferred from the claim text.