Engineered phosphopentomutase variant enzymes
AssigneeCodexis, Inc.
InventorsJonathan Vroom, Santhosh Sivaramakrishnan, Jessica Anna Hurtak
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Abstract
The present invention provides engineered phosphopentomutase (PPM) enzymes, polypeptides having PPM activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing PPM enzymes are also provided. The present invention further provides compositions comprising the PPM enzymes and methods of using the engineered PPM enzymes. The present invention finds particular use in the production of pharmaceutical compounds.
Claims11
An engineered phosphopentomutase comprising a polypeptide sequence having at least 95%, 96%, 97%, 98%, 99%, or more sequence identity to SEQ ID NO:
- 4, and/or 12 or a functional fragment thereof, wherein the polypeptide sequence of said engineered phosphopentomutase comprises the substitution set 22/188/257/266/341 and wherein the amino acid positions of said polypeptide sequence are numbered with reference to SEQ ID NO:
- 4.
Dependencies are inferred from the claim text.