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US 12,668,788 B2Granted
Asx-specific protein ligases and uses thereof
AssigneeNanyang Technological University
InventorsJames P. Tam, Julien Lescar, Muxinya He, Abbas El Sahili, Chuan Fa Liu, Side Hu
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Abstract
The present invention lies in the technical field of enzyme technology and specifically relates to enzymes having Asx-specific ligase and cyclase activity and to nucleic acids encoding those as well as methods of the manufacture of said enzymes. The enzymes having Asx-specific ligase and cyclase are isolated from plants of the Violaceae family. Further encompassed are methods and uses of these enzymes.
Claims9
A recombinant polypeptide having protein ligase activity comprising:
- (i) the amino acid sequence as set forth in SEQ ID NO:
- 1;
- or
- (ii) an amino acid sequence of at least 90% identity with SEQ ID NO:1 and comprises:
- (a) the amino acid residue W or Y at position 195, the amino acid residue I, C, A or V at position 196, and the amino acid residue T, A or V at position 197 of SEQ ID NO:1;
- (b) the amino acid residue A or G at position 126 and, the amino acid A or P at position 127 of SEQ ID NO:1, but not GP at positions 126 and 127 of SEQ ID NO:
- 1;
- and
- (c) at least two of the amino acid residues selected from:
- the amino acid residue N at position 19 of SEQ ID NO:
- 1;
- the amino acid residue H at position 124 of SEQ ID NO:
- 1;
- and the amino acid residue C at position 166 of SEQ ID NO:1.
11 more elements
Dependencies are inferred from the claim text.