Preparation method and application of vesicle formed by erythrocyte membrane encapsulating Newcastle disease virus

A preparation method of a vesicle formed by an erythrocyte membrane encapsulating Newcastle disease virus, comprising:

• mixing a compacted erythrocyte solution, a phosphate buffered saline (PBS) solution, and Newcastle disease virus (NDV);
• centrifuging the resulting mixture of erythrocytes added with NDV;
• and
• resuspending the centrifuged pellet of the resulting mixture in a PBS solution to obtain the vesicle formed by the erythrocyte membrane encapsulating Newcastle disease virus;
• wherein the compacted erythrocyte solution is obtained by:
• diluting anticoagulated whole blood with an equal volume of PBS solution, separating erythrocyte, and removing the supernatant by centrifugation;
• and
• adding a PBS solution to wash, and removing a supernatant by centrifugation to obtain the compacted erythrocyte solution;
• wherein step of centrifuging the resulting mixture of erythrocytes added with NDV comprises:
• collecting the mixture of erythrocytes with NDV and add the mixture into a 50 mL centrifuge tube;
• centrifuging at 1300 mm 40° C. for 8 min, and then centrifuging at 5000 mm 40° C. for 15 min;
• transferring the supernatant to another centrifuge tube and centrifuging at 14000 g 4° C. for 2 min;
• and
• transferring the supernatant to another centrifuge tube and centrifuging at 14000 g 4° C. for 1 h;
• wherein the step of resuspending the centrifuged pellet of the resulting mixture in a PBS solution to obtain the vesicle formed by the erythrocyte membrane encapsulating Newcastle disease virus comprises:
• discarding the supernatant, resuspending the centrifuged pellet of the resulting mixture in 1 mL of PBS, and centrifuging at 14000 g 4° C. for 30 min;
• and
• discarding the supernatant, resuspending the centrifuged pellet of the resulting mixture in 1 mL of PBS, and storing at −20° C.