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US 12,668,778 B2Granted
Method for expansion of double negative regulatory T cells
AssigneeUniversity Health Network
InventorsLi Zhang, Paulina Achita, Jongbok Lee, Dalam Ly, Dzana Dervovic
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Abstract
There is provided herein a method for expanding human CD4−CD8− regulatory T cells (DN Tregs) from a population of cells comprising DN Tregs, comprising: culturing the population of cells with artificial antigen presenting cells (APCs), preferably the DN Tregs are αβ-TCR+CD56− or alternatively γδ-TCR+.
Claims23
A method of treating allograft rejection, graft vs. host disease (GVHD), or an autoimmune disease in a subject, comprising:
- expanding human CD4−CD8− regulatory T cells (DN Tregs) from a population of cells comprising DN Tregs, by:
- depleting the population of cells comprising DN Tregs of CD4+ and CD8+ cells;
- activating the depleted population of cells with anti-CD3 antibodies;
- then
- culturing the activated population of cells with artificial antigen presenting cells (APCs) that express at least one cell surface adhesion molecule for immunological synapse, the at least one cell surface adhesion molecule comprising at least one of CD54 and CD58;
- wherein the DN Tregs are αβ-TCR+CD56−, γδ-TCR+, or both;
- and
- administering to the subject a therapeutically effective amount of the expanded DN Tregs.
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