Production method for organoid
AssigneesJsr Corporation, Keio University
InventorsKentaro Shoji, Toshiro Sato
Life of this patent
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Abstract
A production method for an organoid, the production method including a step of culturing adult stem cells or a cell tissue piece including adult stem cells in a medium containing a chimeric Fibroblast Growth Factor (FGF) that includes a partial region of FGF1 and a partial region of FGF2; an organoid produced by the production method; a medium including a chimeric FGF and having a content of chimeric FGF of 50 ng/mL or less; and an evaluation method for a test substance are provided, and according to the chimeric FGF, a content of growth factors included in a medium can be reduced.
Claims13
A production method for an organoid, the method comprising:
- culturing adult stem cells or a stem cell tissue piece including adult stem cells in a medium comprising a chimeric Fibroblast Growth Factor (FGF) having the amino acid sequence of SEQ ID NO:
- 2, SEQ ID NO:
- 3, or SEQ ID NO:
- 4.
A medium comprising a chimeric FGF,
- wherein the chimeric FGF is a protein having the amino acid sequence of SEQ ID NO:
- 2, SEQ ID NO:
- 3, or SEQ ID NO:
- 4, and
- wherein a content of the chimeric FGF is 50 ng/ml or less.
A chimeric FGF, wherein the chimeric FGF is a protein having the amino acid sequence of SEQ ID NO:
- 2, SEQ ID NO:
- 3, or SEQ ID NO:
- 4.
Dependencies are inferred from the claim text.