Cell culture device
AssigneeNational Taiwan University
InventorsYu-Hsiang Hsu, Hong-Wen Wang
Life of this patent
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Abstract
A cell culture device is provided, which comprises a cavity and a base layer, wherein the base layer is a type of plastic thin film and has a thickness of 1 μm to 100 μm; a second-moment of inertia lower than 6×106 μm4; and a resultant flexural rigidity of 1×10−6 Pa·m4 to 0.02 Pa·m4. Accordingly, the base layer can produce an out-of-plane strain, and bending deformation can occur. Therefore, a growth space close to in vivo environment is provided by the base layer for the cells when the cells attach to the base layer, thereby promoting the growth and maturation of the cells and tissues.
Claims5
A cell culture device, comprising:
- a cavity including an opening and a bottom;
- a base layer disposed at the bottom of the cavity and closing the bottom, wherein a thickness of the base layer is between 1 μm to 100 μm, a second-moment of inertia of the base layer is lower than 6×106 μm4, and a resultant flexural rigidity of the base layer is between is 1×10−6 Pa·m4 to 0.02 Pa·m4;
- and
- a hollow spacer disposed under the base layer and contacting with an edge of the base layer;
- wherein the hollow spacer is configured to prevent the base layer from contacting any surface thereunder and allows the base layer to generate out-of-plane strain during a cell culture process;
- wherein the base layer is made of a plastic film at least one selected from the group consisting of polymethyl methacrylate, polypropylene, polystyrene, polyethylene, polyethylene terephthalate, polyvinyl chloride, polydimethylsiloxane, polyurethane, polyacrylamide, and mixtures thereof;
- wherein the base layer further includes a plurality of microgrooves, wherein each one of the plurality of microgrooves are arranged adjacent and parallel to each other.
Dependencies are inferred from the claim text.