Microfluidic system simulating lung tissue

A microfluidic control method in a microfluidic system simulating a lung tissue, the system comprising:

• a first layer;
• a second layer;
• a third layer;
• a first chamber for gas perfusion between the first layer and the second layer;
• and
• a second chamber for medium-containing fluid perfusion between the second layer and the third layer,
• wherein the second layer includes a porous membrane,
• the porous membrane includes lung epithelial cells, lung fibroblasts, and vascular endothelial cells,
• the lung epithelial cells face the first chamber,
• the vascular endothelial cells face the second chamber;
• the method comprising
• a microfluidic perfusion step of perfusing gas to the first chamber of the microfluidic system and perfusing the medium-containing fluid to the second chamber,
• wherein the porous membrane includes the lung epithelial cells that are seeded and cultured on one side of the porous membrane, and the porous membrane includes the lung fibroblasts and the vascular endothelial cells that are seeded and cultured on the opposite side of the porous membrane,
• wherein the lung epithelial cells are alveoli epithelial cells, and
• wherein the first layer comprises one or more gas partial pressure sensors and the third layer comprises one or more pH sensors;
• a step of adjusting a pressure of a perfusion gas for simulating respiration movement;
• and
• a step of measuring pH by the pH sensor of the system and measuring a gas partial pressure by the gas partial pressure sensor.