Cartridge for sample preparation and molecule analysis, cartridge control machine, sample preparation system and method using the cartridge

A device, comprising:

• a cartridge for sample preparation and molecule analysis, the cartridge including:
• a body including:
• a first main face and a second main face opposite to the first main face;
• a first sidewall extends from the first main face to the second main face;
• a second sidewall extends from first main face to the second main face, the second sidewall is opposite to the first sidewall;
• a third sidewall extends from the first main face to the second main face, the third sidewall is opposite to the first sidewall;
• an inlet at the second sidewall and an outlet at the third sidewall;
• a waste chamber;
• a plurality of reagent chambers separated from the waste chamber by a plurality of first partition walls, each one of the plurality of reagent chambers is separated from an adjacent one of the plurality of reagent chambers by one of the plurality of first partition walls;
• and
• an analysis recess separated from waste chamber by a second partition wall, the analysis recess extends into the second main face, and the analysis recess has a parallelepipedal shape having rounded corners at an end of the analysis recess;
• a first closing wall coupled to the first main face;
• a second closing wall coupled to the second main face, the second closing wall including a chip receiving opening, and the second closing wall including one or more first sidewalls that define and delimit the chip receiving opening;
• and
• a chip within the chip receiving opening, the chip is coupled to the second main face, the chip fully overlaps the analysis recess, the chip includes a first surface that closes off sides of the analysis recess, the chip including one or more second sidewalls that abut the one or more first sidewalls, the one or more second sidewalls of the chip are spaced outward from the sides of the analysis recess, and the first surface of the chip and the sides of the analysis recess delimit and define an analysis chamber such that the first surface of the chip is exposed to an analysis sample when present within the analysis chamber.

A device, comprising:

• a cartridge for sample preparation and molecule analysis, the cartridge including:
• a body including:
• a first side;
• a second side opposite to the first side;
• an extraction recess that extends into the second side;
• an analysis recess that extends into the second side, the analysis recess includes a parallelepipedal shape and an end having rounded corners;
• a waste opening that extends into the second side to the first side, the waste opening extends entirely through the body;
• and
• one or more valve holes that extend into the second side to the first side, the one or more valve holes extend entirely through the body;
• a first closing wall coupled to the first side of the body, the first closing wall including:
• a waste recess in fluid communication with the waste opening;
• a first gasket cavity;
• and
• a second gasket cavity;
• a first gasket in the first gasket cavity;
• a second gasket in the second gasket cavity;
• a second closing wall coupled to the second side of the body, the second closing wall including a chip receiving opening, and the second closing wall including one or more first sidewalls that define and delimit the chip receiving opening;
• and
• a chip within the chip receiving opening, the chip is aligned with and overlaps the analysis recess, the chip is coupled to the second side of the body, the chip includes a heating and temperature control element, configured to, in operation, heat a solution in the analysis recess, and the chip closes off sides of the analysis recess delimiting an analysis chamber with the analysis recess and the chip,
• one or more second sidewalls of the chip abut the one or more first sidewalls of the second closing wall, and the one or more second sidewalls of the chip are spaced outward from the sides of the analysis recess, and a first surface of the chip and the sides of the analysis recess delimit the analysis chamber, and the first surface of the chip is exposed to the solution when in the analysis recess.