Device and method for accelerated material extraction and detection
AssigneeWisconsin Alumni Research Foundation
InventorsDavid J. Beebe, Duane S. Juang, Terry D. Juang, Thomas C. Friedrich, David H. O'Connor
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Abstract
A method and device are provided for detecting a target in a biological sample. The target binds to a solid phase substrate. First and second cavities in a plate are filled with an oil. The first and second cavities are in fluid communication with each other. The solid phase substrate is magnetically drawn sequentially from a drop of the biological sample in the first cavity, through the oil, into a drop of the reaction solution in the second cavity. A change in a parameter of the drop of the reaction solution indicates the presence of the target.
Claims17
A device for detecting a target in a biological sample, the target being connectable to a solid phase substrate, the device comprising:
- a plate having upper and lower surfaces and including:
- first, second and third spaced wells in the plate, each of the first, second and third spaced wells defining corresponding cavities which are fluidly connected and have dimensions;
- a first collar projecting from the upper surface of the plate and defining a non-circular opening in communication with the cavity defined by the second well, the first collar configured to prevent a pipetting mechanism from engaging a bottom surface partially defining the cavity defined by the second well;
- a magnetic manipulation plate including an upper surface defining a microfluidic plate receipt cavity for removably receiving the plate therein, the plate slidable within the microfluidic plate receipt cavity between a first binding position, a second position and a detection position;
- and
- a magnet force generator for generating a magnetic force for attracting the solid phase substrate;
- wherein:
- the magnet force generator is axially aligned with the cavity defined by the first well with the plate in the first binding position;
- the magnet force generator is axially aligned with the cavity defined by the second well with the plate in the second position;
- and
- the magnet force generator is axially aligned with the cavity defined by the third well with the plate in the third detection position.
A device for detecting a target in a biological sample, the target being connectable to a solid phase substrate, the device comprising:
- a plate having upper and lower surfaces and including:
- first, second and third spaced wells in the plate, each of the first, second and
- third spaced wells defining corresponding cavities which are fluidly connected and
- have dimensions;
- a first channel interconnecting the cavity defined by the first well with the cavity defined by the second well;
- a second channel interconnecting the cavity defined by the second well with the cavity defined by the third well;
- and
- a first collar projecting from the upper surface of the plate and defining a non-circular opening in communication with the cavity defined by the second well, the first collar configured to prevent a pipetting mechanism from depositing a fluid directly into the first and second channels;
- and
- a magnet force generator for generating a magnetic force for attracting the solid phase substrate, one of the magnetic force generator and the plate being movable between a first position wherein the magnetic force generator is aligned with the first well, a second position wherein the magnetic force generator is aligned with a second well, and a third position wherein the magnetic force generator is aligned with the third well;
- wherein:
- the well surfaces include a bottom surface;
- and
- the first collar is configured to prevent the pipetting mechanism from engaging the bottom surface of the second well.
Dependencies are inferred from the claim text.