Stabilized mutants of quorum quenching lactonase and use thereof in treatment of pathogens
AssigneeMigal Galilee Research Institute Ltd.
InventorsLivnat Afriat-Jurnou, Maayan Erov, David Gurevich, Mery Dafny Yelin
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Abstract
Mutated phosphotriesterase-like lactonases or functional fragments can be used in methods for treating or preventing infection of a bacterium in a host, such as a plant or a part, organ or a plant propagation material. The methods include applying the mutated phosphotriesterase-like lactonases or the wild-type enzyme to the host Cells expressing the mutated phosphotriesterase-like lactonases can also be produced using nucleic acid molecules and vectors encoding the mutated phosphotriesterase-like lactonases or functional fragments.
Claims27
A mutated phosphotriesterase-like lactonase having at least 90% identity with the wild-type putative parathion hydrolase from M. tuberclorosis of SEQ ID NO:
- 1, in which an amino acid residue corresponding to position 59 or 172 of SEQ ID NO:
- 1 is substituted, wherein a glycine residue corresponding to G59 is substituted by an amino acid residue selected from valine, alanine, leucine, and isoleucine, or a histidine residue corresponding to H172 is substituted by an amino acid residue selected from tyrosine, phenylalanine and tryptophan, and said mutated phosphotriesterase-like lactonase has substantially identical TIM-barrel fold to a wild-type phosphotriesterase-like lactonase and preserves catalytic residues in its active site.
Dependencies are inferred from the claim text.