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US 12,667,099 B2Granted
Cell preservation method
AssigneeCell Exosome Therapeutics Inc.
InventorsYasutomo Yanagita, Chugo Rinoie, Takefumi Ishidao, Itsunari Minami
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AssigneeCell Exosome Therapeutics Inc.
InventorsYasutomo Yanagita, Chugo Rinoie, Takefumi Ishidao, Itsunari Minami
Estimating the term…
The purpose of the present invention is to obtain a cell suspension with a low concentration of cryoprotectant. This method of preserving cells used comprises the steps of (a) enriching cells from a cell suspension containing the cells and a cryoprotective solution to generate an enriched fraction, and (b) freezing the enriched fraction to prepare a frozen material. It is also possible to use a method of producing a cell suspension, comprising the steps of (a) enriching cells from a cell suspension containing the cells and a cryoprotective solution to generate an enriched fraction, (b) freezing the enriched fraction to prepare a frozen material, (c) thawing the frozen material to prepare a thawed material, and (d) mixing the thawed material and a solution to produce a cell suspension.
A bag containing a composition containing stem cells and a cryoprotectant, wherein the composition forms a cell pellet over a side within the bag.
A method of producing a stem cell suspension for a method of regenerative medicine, comprising the steps of thawing a frozen composition forming a cell pellet over a side within a bag, wherein the cell pellet contains a frozen cryoprotectant and a frozen single cell population of 2.0×107 cells/ml or more cells to obtain a thawed composition, and adding a solution into the bag containing the thawed composition to produce a stem cell suspension.
Dependencies are inferred from the claim text.