Capture construct and method for detecting a plurality of analytes

A capture construct for capturing a plurality of analytes of a biological sample, the capture construct comprising:

• a nanostructure backbone,
• at least a first orientation indicator and a second orientation indicator, wherein the first orientation indicator and the second orientation indicator are spaced apart from each other, or arranged on opposite ends of the nanostructure backbone,
• at least a first plurality of capture regions on the nanostructure backbone, each capture region comprising at least one affinity capture reagent, configured to capture one of the plurality of analytes, and
• at least one first affinity reporter reagent comprising a first reporter tag and configured to attach to an analyte of the plurality of analytes, wherein the first reporter tag is optically readable, to determine whether or not the respective analyte is captured by the at least one affinity capture reagent.