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US 12,663,422 B2Granted
Method for detection of CD16b
Assignee52 North Health Ltd
InventorSaif Ahmad
Life of this patent
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Abstract
The invention provides methods of determining the neutrophil level in a sample by determining the level of CD16b, including the intracellular form of CD16b in a sample, such as a blood sample. The methods may further comprise the determination of lactate levels Also provided are associated diagnostic and therapeutic methods. Further provided are kits and devices for performing the methods, particularly lateral flow kits and devices.
Claims17
A method of determining a neutrophil level in a sample from a subject, the method comprising:
- determining the level of CD16b in the sample, wherein the CD16b comprises the intracellular form of CD16b, wherein
- (i) the method further comprises a step of removing at least a proportion, most, or all of the soluble form of CD16b from the sample prior to determining the level of CD16b;
- or
- (ii) the method is performed on a sample from the subject from which at least a proportion, most, or all of the soluble form of CD16b has previously been removed, and
- wherein the step of removing at least a proportion, most, or all of the soluble form of CD16b is a filtration step, wherein the filtration step yields:
- (a) a filtered sample comprising white blood cells, wherein the filtered sample is depleted of the soluble components of the sample;
- and
- (b) a solution comprising the soluble form of CD16b, and
- wherein determining the level of CD16b in the sample comprises assaying the filtered sample comprising white blood cells for CD16b.
6 more elements
Dependencies are inferred from the claim text.