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US 12,663,371 B2Granted
Method of detecting an analyte in a medium comprising a light scattering constituent
AssigneeKing's College London
InventorMark Wallace
Life of this patent
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Abstract
The invention provides methods of optically detecting an analyte in a medium as the analyte moves with respect to a nanopore. The analyte may be, for example, a biological molecule such as a polynucleotide or polypeptide. Systems and apparatuses for carrying our such methods are also provided.
Claims22
A method of detecting an analyte in a medium, the medium comprising a light-scattering constituent, the method comprising:
- contacting the medium with a nanopore;
- generating a flux of the constituent through the nanopore;
- contacting the analyte with the nanopore so that the analyte influences the flux of the constituent through the nanopore;
- illuminating the medium in the vicinity of the nanopore with one or more light sources;
- and
- taking one or more measurements of the light scattered by the constituent in the vicinity of the nanopore as the analyte moves with respect to the nanopore, and thereby detecting the analyte as it moves with respect to the nanopore.
3 more elements
A system, comprising:
- a medium comprising a light-scattering constituent;
- a nanopore in contact with the medium;
- the medium in the vicinity of the nanopore being illuminated by one or more light sources;
- and
- a reference light source.
2 more elements
An apparatus for detecting an analyte, comprising:
- a chamber having a volume, said volume containing a medium comprising a light-scattering constituent;
- a membrane comprising a nanopore, wherein said nanopore is in contact with said medium;
- one or more light sources for illuminating the volume in the vicinity of the nanopore;
- and
- a detector for detecting light scattered by the constituent.
2 more elements
Dependencies are inferred from the claim text.