Multiplexed on-chip impedance cytometry system and method

A system comprising:

• a microfluidic chip comprising a plurality of microfluidic channels configured to receive one or more flowing samples comprising biologic or particle components, each of the plurality of microfluidic channels including a first channel and a second channel, wherein each of the first channel and the second channel includes an upstream microfluidic channel and a plurality of downstream microfluidic channels, wherein each upstream microfluidic channel is connected to a respective plurality of downstream microfluidic channels;
• and
• at least one circuit operatively coupled to the microfluidic chip configured to:
• apply a first set of electric field across a first set of one or more electrodes located in a first channel containing a first sample of the one or more samples to provide an on-chip measured impedance spectra, and
• control polarization and/or manipulation of the biologic or particle components flowing through the second channel based on the on-chip measured impedance spectra by applying a second set of electric field across a second set of one or more electrodes located in a microfluidic channel containing a second sample of the one or more samples to selectively urge the biologic or particle components of the second sample into one or more lanes of a plurality of lanes in a flow to continuously separate the biologic or particle components to channel inlets of the respective plurality of downstream microfluidic channels.