Composition and methods for hybridization
Assignee42 Life Sciences GmbH & Co. KG
InventorsPiere Marggraf-Rogalla, Sven Hauke
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Abstract
The present invention relates to a composition for use in hybridization, the composition comprising: (a) at least one hybridization probe (“component (a)”); (b) at least one polar protic or polar aprotic solvent (“component (b)”) having a cyclic molecule structure; and (c) at least one carboxylic acid amide and/or salts thereof (“component (c)”), in an amount of more than 10 vol %, based on the composition. The invention furthermore relates to a use of this composition, and to methods for detecting nucleic acids and/or chromosomal aberrations in a biological sample by way of hybridization, using the composition.
Claims8
In a composition for detecting nucleic acids and/or chromosomal aberrations in a biological sample via in situ hybridization, the composition comprising at least one fluorescent-labeled locus-specific hybridization probe (“component (a)”) and water as a carrier or excipient, wherein the hybridization probe is selected from DNA, RNA, and locked nucleic acids (LNA), wherein the improvement comprises:
- that the composition comprises:
- 2-pyrrolidone or 2-piperidone (“component (b)”) in an amount of 2 to 13 vol % or wt %, based on the composition, and
- formamide and/or salts thereof (“component (c)”) in an amount of 20 to 30 vol % based on the composition, wherein the composition comprises said component (b) and said component (c) in a volume-based ratio in the range of 1:10 to 1:3, said composition further comprising:
- dextran sulfate (“component (d)”), and
- at least one inorganic salt (“component (g)”) wherein the inorganic salt is sodium chloride.
Dependencies are inferred from the claim text.