Non-invasive gene mutation detection in lung cancer patients

A method of detecting lung cancer in a subject comprising:

• obtaining a sample from the subject;
• mixing a first portion of the sample with a solution comprising a labeled detector probe;
• adding the mixture to a first electrode chip on a device, the electrode chip comprising a working electrode, a counter electrode, and a reference electrode;
• wherein the working electrode is coated with a conducting polymer embedded with a capture probe capable of binding to a marker associated with lung cancer in the sample;
• generating 5 cycles to about 10 cycles of a cyclic square wave electric fields field across the electrode for each surface reaction;
• and
• measuring the current in the electrode chip, wherein a change in current is correlated to the presence of the marker associated with lung cancer in the sample
• wherein the capture and detection probes are selected from the group consisting of
• i) the capture probe comprises the nucleotide sequence of SEQ ID NO:
• 1 and the detector probe comprises the nucleotide sequence of SEQ ID NO:
• 2;
• and
• ii) the capture probe comprises the nucleotide sequence of SEQ ID NO:
• 3 and the detector probe comprises the nucleotide sequence of SEQ ID NO:
• 4.