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US 12,662,685 B2Granted
Self-inactivating transposase plasmids and uses thereof
AssigneeSeattle Children's Hospital
InventorsMichael C. Jensen, Joshua Gustafson, Joseph Cheng, Rachel Wilson, Kamila Sabina Gwiazda, Jeremy Bjelajac
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Abstract
Some embodiments provided herein relate to gene delivery systems and methods using a single plasmid that carries a self-inactivating transposase gene and a corresponding transposon. Some embodiments include nucleic acids having certain sequences, vector including such nucleic acids, and compositions including the vectors.
Claims15
A vector for delivery of a payload into a cell genome, the vector comprising (a), (b), and (c) in a 5′ to 3′ order:
- (a) a first nucleic acid comprising (i) a nucleotide sequence encoding a transposase, and (ii) an intron comprising a first transposase recognition site (TRS), wherein the intron is inserted into the nucleotide sequence encoding the transposase;
- (b) a second nucleic acid encoding the payload;
- and
- (c) a second TRS.
1 more element
Dependencies are inferred from the claim text.