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US 12,662,681 B2Granted
Multi-pulse transfection methods and cells
AssigneeNantBio, Inc.
InventorsMarcos Sixto, Kayvan Niazi, Ting Wu, Chihwei Chang
Life of this patent
Estimating the term…
Abstract
Systems and methods are provided for transfecting immune competent cells with RNA at high efficiency and viability.
Claims17
A method of transfecting mammalian cells with a RNA, comprising:
- disposing the mammalian cells with the RNA in a transfection medium, wherein the mammalian cells are NK cells;
- applying two, three, or four pulses to the mammalian cells at a field strength of 600-1,400 V/cm and a time constant from about 0.5 msec to about 10 msec to produce transfected mammalian cells.
A method of transfecting mammalian cells with a RNA, comprising:
- disposing the mammalian cells with the RNA in a transfection medium, wherein the mammalian cells are NK cells;
- and
- applying two, three, or four pulses to the mammalian cells at a voltage of 150-250 V, a gap width of about 0.1 cm to about 0.4 cm, and a time constant from about 0.5 msec to about 10 msec to produce transfected mammalian cells, wherein the pulses are delivered from a capacitor having a capacitance of between 5-50 μF.
A method of transfecting mammalian cells with a RNA, comprising:
- disposing the mammalian cells with the RNA in a transfection medium, wherein the mammalian cells are NK cells;
- and
- applying two, three, or four pulses to the mammalian cells at a field strength of 600-1,400 V/cm and a time constant from about 0.5 msec to about 10 msec to produce transfected mammalian cells, wherein the pulses are delivered from a capacitor having a capacitance of between 5-50 μF.
Dependencies are inferred from the claim text.