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US 12,662,680 B2Granted
Microorganisms for diterpene production
AssigneesDsm IP Assets B.V., Cargill, Incorporated
InventorCatharina Petronella Antonia Maria Kolen
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AssigneesDsm IP Assets B.V., Cargill, Incorporated
InventorCatharina Petronella Antonia Maria Kolen
Estimating the term…
The invention disclosed herein relates generally to the field of recombinant production of a steviol glycoside, to the field of bioconversion of steviol into a steviol glycoside and to the field of bioconversion of a steviol glycoside into a further steviol glycoside. Particularly, the invention provides a process for recombinant production of a steviol glycoside, a process of bioconversion of steviol into a steviol glycoside, a process for bioconversion of a steviol glycoside into a further steviol glycoside and a composition comprising a steviol glycoside. More particularly, the invention relates to a microorganism that has a deficiency of a serine/threonine protein kinase and comprises a polynucleotide encoding a polypeptide having uridine diphosphate-dependent glucosyltransferase (UGT) activity.
A recombinant yeast comprising one or more polynucleotide(s) encoding one or more polypeptide(s) having uridine diphosphate-dependent glycosyltransferase (UGT) activity, wherein said recombinant yeast comprises a mutation, insertion, substitution, or deletion in a serine/threonine protein kinase gene selected from serine/threonine protein kinase 1 (PSK1) and serine/threonine protein kinase 2 (PSK2) that reduces the kinase activity relative to the corresponding yeast lacking the mutation, insertion, substitution, or deletion.
Dependencies are inferred from the claim text.